Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Pifithrin-β hydrobromide (Cyclic PFT-α) 是一种 p53 的抑制剂,可逆地阻断 p53 依赖性转录激活和细胞凋亡。IC50值为23 μM。
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
2 mg | ¥ 259 | 现货 | ||
5 mg | ¥ 455 | 现货 | ||
10 mg | ¥ 778 | 现货 | ||
25 mg | ¥ 1,620 | 现货 | ||
50 mg | ¥ 2,930 | 现货 | ||
100 mg | ¥ 4,250 | 现货 | ||
1 mL * 10 mM (in DMSO) | ¥ 486 | 现货 |
产品描述 | Pifithrin-β hydrobromide (Cyclic PFT-α) is an inhibitor of p53; reversibly blocks p53-dependent transcriptional activation and apoptosis. Protects against neuronal death in models of stroke and neurodegenerative disorders. Active in vivo; protects mice from the side-effects of Y therapy associated with p53 induction. Supresses self-renewal of embryonic stem cells. Also aryl hydrocarbon receptor (AHR) agonist, causes upregulation of AHR target gene CYP1A1 (EC50 = 1.1 μM). |
靶点活性 | p53:23 μM(EC50) |
体外活性 | PFTα molecule could not take a planar conformation required for AhR activation whereas PFTβ showed a conformation similar to those of the prototypical AhR ligand β-naphthoflavone. In both cell lines, PFTα and PFTβ provoked different responses related with AhR activation. However, when cyclization of PFTα to PFTβ was hampered by acetylation of the exocyclic nitrogen, all these responses were not observed. These results lead to the conclusion that the activation of the AhR is probably caused by PFTβ instead of PFTα. |
别名 | Cyclic PFT-α, PFT-β, Cyclic Pifithrin-α hydrobromide, Pifithrin-β, PFT β (hydrobromide) |
分子量 | 349.29 |
分子式 | C16H17BrN2S |
CAS No. | 511296-88-1 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMSO: 13.75 mg/mL (39.37 mM)
可选溶剂 | 浓度 体积 质量 | 1 mg | 5 mg | 10 mg | 25 mg |
DMSO | 1 mM | 2.863 mL | 14.3148 mL | 28.6295 mL | 71.5738 mL |
5 mM | 0.5726 mL | 2.863 mL | 5.7259 mL | 14.3148 mL | |
10 mM | 0.2863 mL | 1.4315 mL | 2.863 mL | 7.1574 mL | |
20 mM | 0.1431 mL | 0.7157 mL | 1.4315 mL | 3.5787 mL |
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
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